The Live Longer Labs team is back and in full force creating all the C60live products. C60live is created fresh, daily in the Live Longer Labs' Rocky Mountain research facility. Pure Bella Vita will continue to handle all C60live orders and shipments. This enables the research team to focus on what they do best: research. Namaste, Team C60live

C60live Research

Antioxidant research and C60

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Possible Mechanisms of Fullerene C60 Antioxidant Action

BioMed Research International

​Novel mechanism of antioxidant activity of buckminsterfullerene C60 based on protons absorbing and mild uncoupling of mitochondrial respiration and phosphorylation was postulated. In the present study we confirm this hypothesis using computer modeling based on Density Functional Theory. Fullerene's geroprotective activity is sufficiently higher than those of the most powerful reactive oxygen species scavengers. We propose here that C60 has an ability to acquire positive charge by absorbing inside several protons and this complex could penetrate into mitochondria. Such a process allows for mild uncoupling of respiration and phosphorylation. This, in turn, leads to the decrease in ROS production.


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Categories: Assorted Benefits of C60,Antioxidant research and C60

Assorted Benefits of C60

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USA Today: Extra virgin olive oil staves off Alzheimer's, preserves memory, new study shows

Temple University research shows extra-virgin olive oil protects against memory loss, preserves the ability to learn and reduces conditions associated with Alzheimer's disease

Researchers at the college's Lewis Katz School of Medicine found mice with EVOO-enriched diets had better memories and learning abilities compared to the rodents who didn't eat the oil.

The real effect of EVOO appeared in the inner-workings of the mice's brains. Neuron connections in the brain were better preserved in those on an EVOO diet.


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Categories: C60 + oils,Assorted Benefits of C60
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Pristine C60 Fullerenes Inhibit the Rate of Tumor Growth and Metastasis

S.V. Prylutska1,*, A.P. Burlaka2 , Yu.I. Prylutskyy1 , U. Ritter3 , P. Scharff3

Aim: To estimate the impact of C60 fullerene aqueous solution (C60FAS) on the rate of transplanted malignant tumor growth and metastasis. Methods: Lewis lung carcinoma was transplanted into С57Bl/6J male mice. Conventional methods for the evaluation of antitumor and antimetastatic effects have been used. Results: The C60FAS at low single therapeutic dose of 5 mg/kg inhibited the growth of transplanted malignant tumor (antitumor effect) and metastasis (antimetastatic effect): the maximum therapeutic effect was found to be of 76.5% for the tumor growth inhibition; the increase of animal life span by 22% was found; the metastasis inhibition index was estimated as 48%. Conclusion: It was found that water-soluble pristine С60 fullerenes efficiently inhibit the transplanted malignant tumor growth and metastasis.


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Category: Assorted Benefits of C60
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Possible Mechanisms of Fullerene C60 Antioxidant Action

BioMed Research International

​Novel mechanism of antioxidant activity of buckminsterfullerene C60 based on protons absorbing and mild uncoupling of mitochondrial respiration and phosphorylation was postulated. In the present study we confirm this hypothesis using computer modeling based on Density Functional Theory. Fullerene's geroprotective activity is sufficiently higher than those of the most powerful reactive oxygen species scavengers. We propose here that C60 has an ability to acquire positive charge by absorbing inside several protons and this complex could penetrate into mitochondria. Such a process allows for mild uncoupling of respiration and phosphorylation. This, in turn, leads to the decrease in ROS production.


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Categories: Assorted Benefits of C60,Antioxidant research and C60
e

A carboxyfullerine SOD mimetic improves cognition and extends the lifespan of mice

Kevin L. Quick a, Sameh S. Ali a, Robert Arch b, Chengjie Xiong c, David Wozniak d, Laura L. Dugan a,∗

In lower organisms, such as Caenorhabditis elegans and Drosophila, many genes identified as key regulators of aging are involved in either detoxification of reactive oxygen species or the cellular response to oxidatively-damaged macromolecules. Transgenic mice have been generated to study these genes in mammalian aging, but have not in general exhibited the expected lifespan extension or beneficial behavioral effects, possibly reflecting compensatory changes during development. We administered a small-molecule synthetic enzyme superoxide dismutase (SOD) mimetic to wild-type (i.e. non-transgenic, non-senescence accelerated) mice starting at middle age. Chronic treatment not only reduced age-associated oxidative stress and mitochondrial radical production, but significantly extended lifespan. Treated mice also exhibited improved performance on the Morris water maze learning and memory task. This is to our knowledge the first demonstration that an administered antioxidant with mitochondrial activity and nervous system penetration not only increases lifespan, but rescues age-related cognitive impairment in mammals. SOD mimetics with such characteristics may provide unique complements to genetic strategies to study the contribution of oxidative processes to nervous system aging. © 2006 Elsevier Inc. All rights reserved.


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Categories: C60 research results on rats ,Assorted Benefits of C60

C60 + oils

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USA Today: Extra virgin olive oil staves off Alzheimer's, preserves memory, new study shows

Temple University research shows extra-virgin olive oil protects against memory loss, preserves the ability to learn and reduces conditions associated with Alzheimer's disease

Researchers at the college's Lewis Katz School of Medicine found mice with EVOO-enriched diets had better memories and learning abilities compared to the rodents who didn't eat the oil.

The real effect of EVOO appeared in the inner-workings of the mice's brains. Neuron connections in the brain were better preserved in those on an EVOO diet.


Read full article

Categories: C60 + oils,Assorted Benefits of C60
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Update on C60 fullerenes in olive oil | AgingSciences - Anti-Aging Firewalls™

By Vince Giuliano and James P Watson

More than a year has passed since publication of the November 2012 blog entry Buckyballs, health and longevity – state of knowledgeIt will be another year or two before we know whether researchers are capable of reproducing the extraordinary longevity impacts on rats reported in the 2011 publication The prolongation of the lifespan of rats by repeated oral administration of [60] fullerene.  We don’t yet know a number of things we would love to know: 1.  Whether indeed it is verified that “buttered buckyballs” can extend the lives of rats?, 2. if so, can they extend the healthspans and lives of humans?, and 3. exactly what molecular mechanisms would be involved in such lifespan extension?.  Because rats normally live 2+ years, it will be at least another year before we start getting answers to the first question.  Because we live 80+ years it may be decades before we start getting answers to the second question.  But we can continue seriously discussing and speculating about the third question now, and that is the focus of this blog entry.  In particular, a new mechanism is proposed for how C60 can possibly drastically reduce ROS and its pro-aging consequences.


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Category: C60 + oils
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Olive Oil Offers Unique Cardiovascular Protection

By Marsha McCulloch, RD

Olive oil has been pinpointed as a major source of the cardiovascular benefits long associated with a Mediterranean diet.1,2 A recent study of a healthy Mediterranean population showed that olive oil consumption accounted for as much as a 44% reduction in cardiovascular deaths compared to those who didn’t use olive oil.3


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Categories: C60 + oils,Oxidative research and C60
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Extra-Virgin Olive Oil: Super food for pets

What’s the difference between extra-virgin olive oil, and plain olive oil? Does it matter which one I give my pet?


Olive oils are graded according to their extraction process and on the acidity of the pressed oil. Extra-virgin olive oil is extracted from olives using pressure only, a process called cold pressing. “Extra-virgin olive oil has 1% acid. It’s the oil that comes from the first pressing of the olives, and is considered the finest, having the freshest, fruitiest flavor,” says Dr. Timothy Harlan, MD, assistant professor of clinical medicine at Tulane University.


If we’re committed to feeding our pets nutritious foods, it makes sense to try and deliver the best. 


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Category: C60 + oils
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Coconut oil is out. These are the oils you should be using, experts say

Last week, the American Heart Association said coconut oil is unhealthy, reigniting a conversation about saturated fat and leaving some confused about what is healthy. 

"There’s a disconnect between people’s general beliefs and what the data actually show," said Donald Hensrud, medical director of the Mayo Clinic Healthy Living Program. 


Like a gluten-free lifestyle, Hensrud said benefits of coconut oil aren't supported by good data. Coconut oil is high in saturated fat. Current data shows saturated and trans fats raise bad (LDL) cholesterol levels.


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Category: C60 + oils

C60 research results on rats

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The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene

SciVerse ScienceDirect and Elsevier.com

Countless studies showed that [60]fullerene (C(60)) and derivatives could have many potential biomedical applications. However, while several independent research groups showed that C(60) has no acute or sub-acute toxicity in various experimental models, more than 25 years after its discovery the in vivo fate and the chronic effects of this fullerene remain unknown. If the potential of C(60) and derivatives in the biomedical field have to be fulfilled these issues must be addressed. Here we show that oral administration of C(60) dissolved in olive oil (0.8 mg/ml) at reiterated doses (1.7 mg/kg of body weight) to rats not only does not entail chronic toxicity but it almost doubles their lifespan. The effects of C(60)-olive oil solutions in an experimental model of CCl(4) intoxication in rat strongly suggest that the effect on lifespan is mainly due to the attenuation of age-associated increases in oxidative stress. Pharmacokinetic studies show that dissolved C(60) is absorbed by the gastro-intestinal tract and eliminated in a few tens of hours. These results of importance in the fields of medicine and toxicology should open the way for the many possible -and waited for- biomedical applications of C(60) including cancer therapy, neurodegenerative disorders, and ageing.
The prolongation of the lifespan of rats by repeated oral administration of [60]fullerene (PDF Download Available). [accessed Sep 1, 2017].


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Category: C60 research results on rats
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Sub-acute oral toxicity study with fullerence C60 in rats

Mika Takahashi1, Hina Kato1, Yuko Doi2, Akihiro Hagiwara2, Mutsuko Hirata-Koizumi1, Atsushi Ono1, Reiji Kubota3, Tetsuji Nishimura3 and Akihiko Hirose1


To obtain initial information on the possible repeated-dose oral toxicity of fullerene C60, Crl:CD(SD) rats were administered fullerene C60 by gavage once daily at 0 (vehicle: corn oil), 1, 10, 100, or 1,000 mg/kg/day for 29 days, followed by a 14-day recovery period. No deaths occurred in any groups, and there were no changes from controls in detailed clinical observations, body weights, and food consumption in any treatment groups. Moreover, no treatment-related histopathological changes were found in any organs examined at the end of the administration period and at the end of the recovery period. Blackish feces and black contents of the stomach and large intestine were observed in males and females at 1,000 mg/kg/day in the treatment group. There were no changes from controls in the liver and spleen weights at the end of the administration period, but those weights in males in the 1,000 mg/kg/day group increased at the end of the recovery period. Using liquid chromatography-tandem mass spectrometry, fullerene C60 were not detected in the liver, spleen or kidney at the end of the administration period and also at the end of the recovery period. In conclusion, the present study revealed no toxicological effects of fullerene C60; however, the slight increases in liver and spleen weights after the 14-day recovery period may be because of the influence of fullerene C60 oral administration.

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Category: C60 research results on rats
e

A carboxyfullerine SOD mimetic improves cognition and extends the lifespan of mice

Kevin L. Quick a, Sameh S. Ali a, Robert Arch b, Chengjie Xiong c, David Wozniak d, Laura L. Dugan a,∗

In lower organisms, such as Caenorhabditis elegans and Drosophila, many genes identified as key regulators of aging are involved in either detoxification of reactive oxygen species or the cellular response to oxidatively-damaged macromolecules. Transgenic mice have been generated to study these genes in mammalian aging, but have not in general exhibited the expected lifespan extension or beneficial behavioral effects, possibly reflecting compensatory changes during development. We administered a small-molecule synthetic enzyme superoxide dismutase (SOD) mimetic to wild-type (i.e. non-transgenic, non-senescence accelerated) mice starting at middle age. Chronic treatment not only reduced age-associated oxidative stress and mitochondrial radical production, but significantly extended lifespan. Treated mice also exhibited improved performance on the Morris water maze learning and memory task. This is to our knowledge the first demonstration that an administered antioxidant with mitochondrial activity and nervous system penetration not only increases lifespan, but rescues age-related cognitive impairment in mammals. SOD mimetics with such characteristics may provide unique complements to genetic strategies to study the contribution of oxidative processes to nervous system aging. © 2006 Elsevier Inc. All rights reserved.


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Categories: C60 research results on rats ,Assorted Benefits of C60

Cardiovascular & Pulmonary research and C60

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Cardiovascular effects of Fullerene Derivatives: A Review of Current Evidence

Alexander V. Syrensky,1 Elena I. Egorova,2 Ilia V. Alexandrov,2 and Michael M. Galagudza2, 1 V.A. Almazov Research Institute of Cardiology of the Ministry of Health Care and 2 St. Petersburg I.P. Pavlov Federal Medical University, St. Petersburg, Russian Federation


During two last decades, several unique physical and chemical properties of buckminsterfullerene or fullerene C60 have been described. However, much less is known about the effects of fullerenes and their derivatives on biological systems. Evidence is beginning to accumulate that fullerenes may exert influence on different physiological and pathophysiological processes primarily because of their antioxidant effects.


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Category: Cardiovascular & Pulmonary research and C60

Oxidative research and C60

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Oxidative Stress in Chronic Obstructive Pulmonary Disease

American Journal of Respiratory and Critical Care Medicine

JOHN E. REPINE , AALT BAST , IDA LANKHORST , and The Oxidative Stress Study Group

Chronic obstructive pulmonary disease (COPD) is a major worldwide health problem that has an increasing prevalence and mortality (12). Oxidative stress, which can be defined as an increased exposure to oxidants and/or decreased antioxidant capacities, is widely recognized as a central feature of many diseases (34). Considerable evidence now links COPD with increased oxidative stress (56). The purpose of this review is to describe the role and origin of the oxidant–antioxidant disturbances that participate in the development of COPD. Our presentation also addresses ways of assessing the contribution of oxidants and identifies therapeutic approaches that could improve cellular oxidant–antioxidant balance in the lungs of COPD patients.


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Category: Oxidative research and C60
e

Olive Oil Offers Unique Cardiovascular Protection

By Marsha McCulloch, RD

Olive oil has been pinpointed as a major source of the cardiovascular benefits long associated with a Mediterranean diet.1,2 A recent study of a healthy Mediterranean population showed that olive oil consumption accounted for as much as a 44% reduction in cardiovascular deaths compared to those who didn’t use olive oil.3


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Categories: C60 + oils,Oxidative research and C60

Uncategorized

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Amorphous Shungite Carbon: A Natural Medium for the Formation of Fullerines

V. A. Reznikov and Yu. S. Polekhovskiœ


—A comparative analysis of data on the density, porosity, and intermolecular space of high-carbon shungites, graphite, glassy carbon, and C60 fullerite gave an estimate of the fullerene content in the shungite samples which agrees with the values obtained by electrochemical and polar solvent extraction methods. A low yield of fullerenes in the extracts obtained with nonpolar solvents is explained by the high polarity and large adsorption energy of fullerenes and related compounds. © 2000 MAIK “Nauka/Interperiodica”.


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